Equipment Availability
The CMA BÍO-BÍO has several technologically advanced basic workstations, which provide independent services, for broad spectrum of usage in various areas of scientific and technological development.
FT-IR Microscope Spotlight 400 & Spectrum Frontier, Perkin Elmer
The FTIR Spectrum Frontier, coupled to a microscope system Spotlight Model 400, is the last generation system in the study of images, ideal in the areas of materials, pharmaceutical, academic, forensic, biomedical and biomaterials. This system works in the Mid range (MID) and Close Range (NIR), which allows the study of a wide range of compounds. Spotlight 400 works in Transmission modes, reflectance and ATR mode, has a dual operating system and detection DuetTM allowing a significantly increase information obtaining from spectral data and id images. It has a variable resolution of 1.56 nm pixel with ATR, achieving exceptional images of the samples.

  • Total available working range 14,700-350 cm-1 (depends on each mode).
  • NB MCT Type Detectors (NIR Band Mercury, Cadmium, Tellurium) and MB MCT (MID Band Mercury, Cadmium, Tellurium).
  • Ability to obtain images with sampling size (pixel) 1.56 & 6.25 µm (ATR mode) and 6.25, 25 and 50 µm (Image mode). Point Mode adjusts the size and orientation of the scanned area as needed.
  • Controllable spectral resolution between 2 and 64 cm-1.
  • The software that controls the computer and manipulate the result is Spectrum (versión 10.03.06.0100) & SprectrumIMAGE (versión R1.7.1.0401).
Confocal Microscope LSM700 Zeiss
Confocal scanning microscope with a semi motorized inverted stand, a comfortable touch screen that helps to control the filters and objectives as well as the use of focus and adjustments of the observation. The confocal scanning head has 2 high sensitivity detectors and 3 laser lines, which allows the collection of up to 3 different fluorescent markers in various focal planes. The acquisition software Zen 2011 provides a fast and intuitive work allowing visualization of both 3D images and as well images of orthogonal cuts. Furthermore, the software allows the analysis and refinement of each of the channels. This microscope is ideal for quick observation and general analysis of images with markers such as GFP, Alexa Fluor 488 – Cy3, Alexa Fluor 555 – Topro 3, Cy5 – Alexa Fluor 633 among others.

CHARACTERISTICS

Inverted microscope with semi motorized stand, Axio Observer Z1 model.

Filter sets for epifluorescence

UV: Ex. 365 - Beam Splitter FT 395 – Em. BP 445/50 (Example: DAPI)
Green: Ex. BP 450 – 490 - Beam Splitter FT 510 – Em. BP 515 – 565 (Example: FITC – Cy2)
Red: Ex. BP 546/12 - Beam Splitter FT 560 – Em. BP 575 – 640 (Example: Cy3 - Rodhamin).

Laser lines

488 nm , 555 nm and 639 nm.

Objective systems

EC-Plan-Neofluar 10x, NA: 0.3, WD. 5.2 mm; 20x; EC Plan-Neofluar NA: 0.5, WD 2.0; 40x oil DIC, NA: 1.3, WD. 0.21 mm; Plan-Apochromat 63x oil DIC, NA: 1.4, WD. 0.19 mm.
Confocal Spectral and Two Photon Microscope LSM780 NLO Zeiss
This system can produce deep, three-dimensional images of living tissue and structures up to about 0.5 mm thick with subcellular resolution. It is very useful in the study of subcelular structures such as spines and axonal processes in neurons that demand an image with a high signal to noise ratio over a large área or volumen due to wide distribution of neuronal processes.

CHARACTERISTICS

Inverted microscope with fully motorized stand, Axio Observer Z1 model.

Laser lines

Laser lines of 405, 458, 488, 514, 561 and 633 nm. In addition, it has a pulsed laser multiphoton adjustable from 680-1080 nm in step of 1 nm that uses low energy photons (infrared) in extremely short pulses which reduces the damage caused by the beam of conventional laser in biological samples.

Objetives

EC-Plan-Neofluar 10x, NA: 0.3, WD. 5.2mm; 20x; EC Plan-Neofluar NA: 0.5, WD 2.0; 40x oil DIC, NA: 1.3, WD. 0.21 mm; Plan-Apochromat 63x oil DIC, NA: 1.4, WD. 0.19 mm.

Detection system

Comprises a polychromatic detector equipped with 32 GaASP photomultipliers more 2 photomultiplier PMP FL that allows simultaneous sprectral separation and detection up to 10 markers. Furthermore, has a transmitted light detector T-PMT

Filter sets for epifluorescence

UV: Ex. 365 - Beam Splitter FT 395 – Em. BP 445/50 (Example: DAPI) Green: Ex. BP 450 – 490 - Beam Splitter FT 510 – Em. BP 515 – 565 (Example: FITC – Cy2) Red: Ex. BP 546/12 - Beam Splitter FT 560 – Em. BP 575 – 640 (Example: Cy3 - Rodhamine).
Super-Resolution Microscope ELYRA S.1 (SR-SIM) Zeiss
The resolving power of an optical microscope is limited to 200 nm laterally (x-axis) and 700 nm in the axial direction (z-plane) due to the physical constraints imposed by the diffraction of light. Using structured light technology and mathematical/computational analysis, we can exceed these limits, doubling the power of both lateral and axial resolution (100 and 350 nm). Super-resolution microscopy is reached by structured illumination (SIM-SR) with two lasers of 488 and 561 nm, achieving super-resolution in two colors, ideal for fine analysis and study of cell structures as the distribution of stress fibers and microfilaments, colocalization studies of synaptic markers, distribution analysis of trasnporters in the plasma membrane, etc.

  • Stage of inverted microscope fully motorized, model Axio Observer Z1.
  • Laser Lines of 488 y 561 nm.
  • Filter set (dichroic) 77 HE, GFP/mRFP/Alexa 633,
    • EX TBP 483-564+642
    • BS TFT EX 506+582+659
    • EM TBP 526+601+688
  • ObjetiveEC Plan-Neofluar 10x/0.30 (AN) M27, WD=5.2 mm
  • Objetive Plan-Apochromat 63x/1.40 (AN) DIC M27, WD=0.19 mm, oil immersion.
Microscopy system for Laser Micro-disecction LMD 7000, Leica
This technique allows to cut and isolate microscopically a homogeneous piece of a sample from any type of starting heterogeneous tissue. Dissection of the sample is carried out through powerful ultraviolet laser of 349 nm which is controlled by a precise optical system free of mechanical movements. Some of its features are:

  • Fully motorized upright microscope for Brightfield (BF), phase contrast (PH), DIC, fluorescence (FLUO) and Polarized Light (POL).
  • Leica digital camera (1.4 megapixels), captures images of epifluorescence and bright field.
  • Objetives 5x,10x, 20x, 40x y 63x and a 150x for chromosome microdissection. 
  • Power of the laser adjustable allowing cutting of both soft and hard samples.
  • Histological samples may be cut, fixed and / or stained and must be mounted on special types of membrane holders (slides and / or slides frame.
Flow Cytometer Cell Sorter BD FACSAria III
Flow cytometry allows simultaneous analysis and characterization of particles and / or cells and their subpopulations.
The cell sorting equipment BDFACSAria III also allows simultaneous separation and collection of up to 4 populations, with a range of size ranging from 0.2 to 50 µm.

The optical system is composed of two lasers, 488 nm and 633 nm, with the ability to detect 8 colors, 10 parameters (including FSC and SSC). The equipment setup is 5-3 (5 colors which are excited by the laser blue and 3 red) with the following filters: Blue laser (488nm): 780/60 BP, 695/40 BP, 616/23 BP, 585/42, 530/30. Red laser (633nm): 780/60BP, 730/45 BP, 660/20 BP.

Has integrated nozzle 70, 85 and 100 µm, that allows the analysis and separation of the majority of applications.

Quality control has Accudrop CST and assure us that the results are reliable.
Center for Advanced Microscopy CMA Bío-Bío. University of Concepción.
University District, No number. Building Ennio Vivaldi Second Floor. Concepción, Chile.
Phone: (56) 41-2204531, (56) 41-2203479,(56) 41-2661356 - Fax (56) 41-2245975.
contacto@cmabiobio.cl - www.cmabiobio.cl